The rapid advancement of virtual simulation technology has catalyzed transformative changes in clinical practical skills training and assessment. In recent years, extensive research and practical applications leveraging this technology have been conducted both domestically and internationally. To unify, standardize, and promote virtual simulation training and assessment for clinical practice skills and evaluation systems, the Chinese Hospital Association, Committee for Healthcare Simulation has organized a multidisciplinary panel of experts spanning clinical practice, technical development, education, and administrative management. Draw lessons from comprehensive investigation of domestic and foreign technical standards, expert consensus, systematic evaluation, original research and other materials related to medical virtual simulation training and assessment, as well as disease diagnosis and treatment guidelines, management consensus and other literatures, which formulate the Expert Consensus on the Application Technology System Standard of Virtual Simulation for Clinical Practice Skills (hereafter referred to as the Consensus). The Consensus was first drafted and revised, and experts including clinical, technical and management in the field of practical skills training and assessment were invited to review the draft and solicit public opinion through email, virtual meeting and in-person ways. After three rounds of opinion solicitation and expert discussion, the draft was revised to improve the final draft of Consensus. The Consensus was composed of four parts: virtual simulation content design and modeling, management platform interface design, content quality evaluation, data transmission and security. This Consensus was made by fully learning related standards, guidelines, consensus, and clinical research for medical virtual simulation training, as well as comprehensive evaluation opinions from experts, which can provide potential reference for medical colleges, medical or training institutions to carry out virtual simulation training for clinical practice skills.