关键词: 肝肿瘤, 转录因子, 危险因素

Abstract: Objective　To investigate the expression and clinical significance of metastasis associated lung adenocarcinoma transcript 1（MALAT1），a long non-coding RNA，in the plasma of patients with hepatocellular carcinoma（HCC）．Methods　We collected 120 plasma and 120 serum samples from pathologically diagnosed HCC patients admitted to the First Affiliated Hospital of Xinjiang Medical University between April 2015 and April 2017.The expression of MALAT1 in the peripheral blood plasma of the HCC patients was detected by real-time fluorescence quantitative polymerase chain reaction（RT-qPCR）．Serum MMP9 and VEGF levels were detected by enzyme-linked immunosorbent assay.The patients were divided into high and low expression groups with the median value of MALAT1 as the cut point，and the correlations between MALAT1 expression and clinical characteristics as well as the serum MMP9 and VEGF level were analyzed.Results　There were significant differences in tumor stage and metastasis between the MALAT1 high and low expression groups（P<0.05）．Other clinical characteristics including sex，age，Child-Pugh classification，serum AFP level，pathological grade，HBV infection and cirrhosis conditions were not significantly different between the two groups （P>0.05）．Cox multivariate regression analysis showed that age，tumor stage，Child-Pugh classification，metastasis ，plasma MALAT1 expression levelwere independent risk factors affecting the prognosis of HCC patients（P<0.05）．Survival analysis showed that the survival rates in the MALAT1 high and low expression groups were significantly different（P<0.010）．Spearman correlation analysis showed that the expression level of MALAT1 was positively correlated with the levels of MMP9 and VEGF （P<0.010）．Conclusion　Patients with high expression of plasma MALAT1 have a short survival time and are prone to metastasis.

Key words: Liver neoplasms, Transcription factors, Risk factors