Abstract: Background　NK/T-cell lymphoma is a malignant tumor that threatens human health.Its pathogenesis has not yet been clarified.There are few reports on the dynamic monitoring of NK/T-cell lymphoma at the molecular level.Objective　To study the application of In Vivo Imaging Technique （IVIT） in the dynamic monitoring of NK/T cell lymphoma growth.Methods　From October 2015 to October 2016，a total of 3 BALB/C-nu grade SPF mice were taken for experiment（The experiment was repeated three times）．100 μl NK92-Luc cell lymphoma （containing 1×106 cells） was subcutaneously injected into the left hypochondrium of each mouse to establish a nude mouse model of subcutaneous xenograft tumor.Tumor volume was measured and calculated every day after the appearance of tumor，and growth curve was drawn according to the tumor growth.Bioluminescence imaging of tumors was detected by small animal in vivo imaging system on the 5th，8th，and 11th days after injection，and curve was plotted according to tumor luminescence photon number.The mice were sacrificed at the end of the experiment，and tumors and livers were taken out and were then embedded into wax blocks of paraffin，and were stained with HE for observing the histomorphological status.Results　All the mice were successfully inoculated with tumors.The mean tumor size at the 5th，8th，and 11th days after injection was（0.003 8±0.002 3），（0.132 3±0.060 7），（0.423 2±0.069 6） mm3，respectively.Tumor growth curve plotted based on the changes in tumor volume showed that the growth of tumor was not obvious in the first 2 days，but quickened significantly in the 3rd and 5th days，and grew fastest in the 6th and 8th days，but tended to be moderate in the 9th to 11th days.The in vivo imager detected that the measured average photon number of the tumor at the 5th，8th，and 11th days after injection was （53 688 222±25 446 363）ph/s，（2 072 443 366±1 029 688 415）ph/s，（5 559 915 605±708 457 376）ph/s，respectively.The tumor growth curve plotted according to tumor luminescence photon number mapping was consistent with the that based on changes in the tumor volume.Light microscopy revealed that the tumor cells were arranged in a sheet or nest shape，and the tumor cells infiltrated the blood vessels and pathological mitotic figures.Conclusion　IVIT can non-invasively，continuously，quantitatively and dynamically monitor nude mice model of NK/T cell lymphoma stably expressing luciferase，suggesting that it may be used for early detection of NK/T cell lymphoma.

Key words: Lymphoma, In vivo imaging technique, NK/T lymphoma, Growth monitoring, Rats

摘要： 背景　NK/T细胞淋巴瘤作为威胁人类生命健康的恶性肿瘤，其发病机制至今尚未明确，从分子水平动态监测NK/T细胞淋巴瘤的相关研究鲜有报道。目的　研究活体成像技术（IVIT）在NK/T细胞淋巴瘤肿瘤生长动态监测中的应用价值。方法　2015年10月—2016年10月，取3只SPF级BALB/C-nu裸鼠进行实验（实验重复3次），按照每只100 μl NK92-Luc淋巴瘤细胞悬液（含1×106个细胞）的剂量，在3只BABL/C-nu裸鼠左侧季肋皮下接种肿瘤细胞，建立裸鼠皮下移植瘤模型。测量并计算肿瘤体积，绘制肿瘤生长曲线；在接种瘤细胞第5、8、11天通过小动物活体成像仪观察肿瘤生物发光成像情况，根据肿瘤发光光子数绘制肿瘤生长曲线；实验结束后断颈法处死裸鼠，取肿瘤、肝脏组织制成蜡块进行苏木素-伊红（HE）染色行组织形态学观察。结果　3只BABL/C-nu裸鼠均成功接种肿瘤细胞，在接种肿瘤细胞第5、8、11天肿瘤大小分别为（0.003 8±0.002 3）、（0.132 3±0.060 7）、（0.423 2±0.069 6）mm3。根据肿瘤体积绘制的肿瘤生长曲线显示，肿瘤体积在前2 d增长不明显，在第3~5天增长明显加快，在第6~8天增长速度最快，在第9~11天增长趋向缓和。第5、8、11天肿瘤平均实测光子数为（53 688 222±25 446 363）、（2 072 443 366±1 029 688 415）、（5 559 915 605±708 457 376）ph/s；根据肿瘤发光光子数绘制的曲线与肿瘤生长曲线变化趋势一致。光镜下见肿瘤细胞排列成片状或巢状，可见肿瘤细胞浸润血管现象和病理性核分裂象。结论　IVIT可无创、连续、定量、动态监测稳定表达荧光素酶的NK/T细胞淋巴瘤裸鼠模型，提示IVIT可用于临床NK/T细胞淋巴瘤的早期发现。

关键词: 淋巴瘤, 活体成像技术, NK/T淋巴瘤, 生长动态监测, 鼠