Abstract: Objective　To explore the expression and influence factors of Klf4 among children with neuroblastoma（NB）.Methods　33 children with NB who were treated in Department of Pediatrics of the Fourth Hospital of Hebei Medical University from January 2007 to March 2013,were selected as the case group,and 10 children with serious hydronephrosis in this hospital during the same period,were selected as the control group.The clinical data of two groups of children were recorded,the pathological tissues of the case group and the residual normal adrenal tissues of the control group were used as study subjects,the method of immunohistochemical SP was used to detect Klf4 expression.The expression intensity of Klf4 was evaluated according to the staining intensity and the percentage of Klf4 positive cells.Results　10 cases（30.3%） in the case group had positive expression of Klf4,and 9 cases（9/10） in the control group had positive expression of Klf4,and the positive rate of Klf4 expression in the case group was significantly lower than that in the control group（χ2=8.801，P=0.003）.In the case group,there was no significant difference in the positive rate of Klf4 expression among children with different sex,age and pathological stages（P>0.05）.In the case group,the positive rate of Klf4 expression among children with ganglioneuroblastoma was significantly higher than that among children with neuroblastoma,and the positive rate of Klf4 expression among children who underwent preoperative chemotherapy was significantly higher than that among children who did not underwent preoperative chemotherapy（P<0.05）.Conclusion　The level of Klf4 expression among children with NB is low,and level of Klf4 expression is related to NB classification.Preoperative chemotherapy drugs may influence the expression of Klf4.

Key words: Neuroblastoma, Klf4, Child

摘要： 目的　探讨儿童神经母细胞瘤（NB）Klf4表达情况及其影响因素。方法　选取2007年1月—2013年3月于河北医科大学第四医院儿科治疗的NB患儿33例为病例组，另选取同期本院严重肾盂积水患儿10例为对照组。记录两组患儿临床资料，取病例组病理组织，对照组残余的正常肾上腺组织，采用免疫组化SP法检测Klf4表达情况，根据染色强度和Klf4阳性细胞所占百分比评价Klf4表达强度。结果　病例组Klf4表达阳性10例（30.3%），对照组Klf4表达阳性9例（9/10），病例组Klf4表达阳性率低于对照组（χ2=8.801，P=0.003）。病例组不同性别、年龄、病理分期患儿Klf4表达阳性率比较，差异均无统计学意义（P>0.05）。病例组节细胞-神经母细胞瘤患儿Klf4表达阳性率高于神经母细胞瘤患儿，术前化疗患儿Klf4表达阳性率高于术前未化疗患儿（P<0.05）。结论　NB患儿Klf4表达下降，且与NB分型有关，术前化疗药物可能对Klf4的表达产生影响。

关键词: 神经母细胞瘤, Klf4, 儿童