中国全科医学 ›› 2022, Vol. 25 ›› Issue (14): 1700-1706.DOI: 10.12114/j.issn.1007-9572.2022.0031

• 论著 • 上一篇    下一篇

不同分期支气管哮喘患者血清生物标志物的比较研究

杨江1, 屠新敏1, 赵欢欢1, 林晓红1, 谢洋1,2, 李素云1,2, 王明航1,2,*()   

  1. 1. 450046 河南省郑州市,河南中医药大学
    2. 450000 河南省郑州市,河南中医药大学第一附属医院
  • 收稿日期:2021-12-05 修回日期:2022-02-10 出版日期:2022-03-31 发布日期:2022-04-07
  • 通讯作者: 王明航
  • 杨江,屠新敏,赵欢欢,等.不同分期支气管哮喘患者血清生物标志物的比较研究[J].中国全科医学,2022,25(14):1700-1706. [www.chinagp.net]
    作者贡献:杨江进行文章的构思与设计,结果的分析与解释,撰写论文;李素云、王明航进行研究的实施与可行性分析;谢洋负责文章的质量控制及审校;屠新敏、赵欢欢、林晓红进行数据收集;赵欢欢、林晓红进行数据整理及录入;杨江、屠新敏进行统计学处理;杨江、王明航对文章整体负责。
  • 基金资助:
    国家自然科学基金资助项目(81873278)

A Comparative Study of Levels of Serum Biomarkers in Patients with Different Stages of Asthma

Jiang YANG1, Xinmin TU1, Huanhuan ZHAO1, Xiaohong LIN1, Yang XIE1,2, Suyun LI1,2, Minghang WANG1,2,*()   

  1. 1. Henan University of Chinese Medicine, Zhengzhou 450046, China
    2. The First Affiliated Hospital of Henan University of Chinese Medicine, Zhengzhou 450000, China
  • Received:2021-12-05 Revised:2022-02-10 Published:2022-03-31 Online:2022-04-07
  • Contact: Minghang WANG
  • About author:
    YANG J, TU X M, ZHAO H H, et al. A comparative study of levels of serum biomarkers in patients with different stages of asthma[J]. Chinese General Practice, 2022, 25 (14) : 1700-1706.

摘要: 背景 哮喘是一种复杂的异质性疾病,频繁急性发作严重影响患者生活质量,并造成沉重的经济负担。近年来,血清生物标志物的发展为实现哮喘精准化治疗和难治性哮喘的诊治提供了新思路。 目的 分析不同分期哮喘患者与健康人群血清生物标志物的分布差异,探究血清生物标志物识别急性发作的潜能。 方法 采用横断面研究,以2018年11月至2020年12月河南中医药大学第一附属医院收治的293例哮喘患者为研究对象,根据哮喘分期分为急性发作期组155例,非急性发作期组138例;同期在医院体检中心招募健康志愿者39例,纳入健康志愿者组。运用ELISA法分别检测三组的血清生物标志物〔白介素4(IL-4)、白介素6(IL-6)、白介素8(IL-8)、白介素17(IL-17)、干扰素γ(IFN-γ)、转化生长因子β2(TGF-β2)、肿瘤坏死因子α(TNF-α)、总免疫球蛋白E(TIgE)、特异性免疫球蛋白E(SIgE)、嗜酸性粒细胞趋化因子(Eotaxin)、嗜酸性粒细胞阳离子蛋白(ECP)、骨膜蛋白(Periostin)、白三烯D4(LTD4)、高迁移率族蛋白B1(HMGB1)、几丁质酶3样蛋白1(YKL-40)〕水平并比较。 结果 急性发作期组、非急性发作期组血清IL-4、IL-6、IL-8、IL-17、TGF-β2、TNF-α、HMGB1、TIgE、SIgE水平均高于健康志愿者组(P<0.05);急性发作期组、非急性发作期组血清IFN-γ水平低于健康志愿者组(P<0.05);急性发作期组血清Eotaxin、YKL-40水平高于非急性发作期组和健康志愿者组,非急性发作期组Eotaxin、YKL-40水平高于健康志愿者组(P<0.05);非急性发作期组Periostin水平低于健康志愿者组(P<0.05)。 结论 血清IL-4、IL-6、IL-8、IL-17、IFN-γ、TGF-β2、TNF-α、SIgE、TIgE、HMGB1与哮喘疾病可能相关,但其表达水平可能与疾病分期无关。Eotaxin、YKL-40可能具有识别哮喘急性发作的潜能。

关键词: 支气管哮喘, 不同分期, 血清, 生物学标记, 呼吸系统疾病

Abstract:

Background

Asthma is a complex heterogeneous disease, whose frequent acute attacks seriously affect the quality of life of patients, and cause a heavy economic burden. The recent development of serum biomarkers provides a new idea for accurate treatment of asthma and diagnosis and treatment of refractory asthma.

Objective

To analyze the difference of serum biomarkers between different stages of asthma patients and healthy people, and to explore the predictive potential of different serum biomarkers for acute asthma attack.

Methods

A cross-sectional design was used. Participants were recruited from the First Affiliated Hospital of Henan University of Chinese Medicine during November 2018 to December 2020, including 293 asthma patients (155 in the acute attack stage, and 138 in non-acute attack stage) , and 39 healthy volunteers from physical examinee center of this hospital. ELISA was used to detect the contents of serum biomarkers〔interleukin 4 (IL-4) , interleukin 6 (IL-6) , interleukin 8 (IL-8) , interleukin17 (IL-17) , interferon-γ (IFN-γ) , transforming growth factor-β2 (TGF-β2) , tumor necrosis factor-α (TNF-α) , total immunoglobulin E (TIgE) , specific immunoglobulin E (SIgE) , eosinophil-selective chemokine (Eotaxin) , eosinophil cationic protein (ECP) , periostin, leukotriene D4 (LTD4) , high mobility group protein B1 (HMGB1) , chitinase 3-like protein 1 (YKL-40) 〕.

Results

Compared with healthy volunteers, both acute and non-acute attack asthma patients had higher levels of serum IL-4, IL-6, IL-8, IL-17, TGF-β2, TNF-α, HMGB1, TIgE and SIgE, and lower level of serum IFN-γ (P<0.05) . The levels of serum Eotaxin and YKL-40 in acute attack asthma patients were higher than those in non-acute attack asthma patients and healthy volunteers (P<0.05) . The levels of Eotaxin and YKL-40 in non-acute attack asthma patients were higher than those in healthy volunteers (P<0.05) . In addition, the level of periostin in non-acute attack asthma patients was lower than that in healthy volunteers (P<0.05) .

Conclusion

Serum IL-4, IL-6, IL-8, IL-17, IFN-γ, TGF-β2, TNF-α, SIgE, TIgE, HMGB1 may be related to asthma, but their expression levels may not be related to disease stage. Eotaxin and YKL-40 may be potential predictors of the acute attack of asthma.

Key words: Asthma, Different stages, Serum, Biomarker, Respiratory tract diseases