关键词: 椎间盘退行性变, 腰椎, 壮腰通络方, 药理作用分子作用机制, 超高效液相色谱-四极杆飞行时间串联质谱, 网络药理学

Abstract: Background　Zhuangyaotongluo decoction is effective for lumbar intervertebral disc degeneration（LIDD），but its chemical constituents and mechanism of action are unclear. Objective　To ascertain the major chemical constituents of Zhuangyaotongluo decoction，and to explore its potential mechanism of action in delaying the progression of LIDD. Methods　In 2020，Ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry（UPLC-Q-TOF-MS/MS） was used to analyze the major chemical constituents of Zhuangyaotongluo decoction，and network pharmacology with mass spectrographic analysis was used to ascertain its potential mechanism of action targets. Therapeutic targets for LIDD were collected via checking 5 therapeutic target databases，and the common ones were identified by using Venn diagram analysis. The protein-protein interaction（PPI） networks of these common targets were obtained from the STRING 11.0 database，and the core targets were extracted from them. Finally，GO enrichment analysis and Kyoto Encyclopedia of Genes and Genomes（KEGG） were used for analyzing the core targets. Results　Zhuangyaotongluo decoction was found to be composed of 129 chemical constituents by UPLC-Q-TOF-MS. Then 96 common targets were obtained by mapping 203 action targets of Zhuangyaotongluo decoction to therapeutic targets for LIDD. PPI network analysis found that AKT1，INS，IL-6，FOS，and CASP3 were core targets. The GO analysis identified 1 022 biological process information，28 cellular components information，and 51 molecular function information. Analysis using KEGG identified 98 related signaling pathways，mainly including HIF-1 signaling pathway，JAK-STAT signaling pathway，Relaxin signaling pathway，p53 signaling pathway，and MAPK signaling pathway. Conclusion　This study revealed the chemical constituents contained in Zhuangyaotongluo decoction and their action targets，biological pathways and mechanisms of action for delaying LIDD，providing a reference basis for future in-depth analysis of its therapeutic substance basis and mechanisms of action.

Key words: Intervertebral disc degeneration, Lumbar vertebrae, Zhuangyaotongluo decoction, Molecular mechanisms of pharmacological action, Ultra performance liquid chromatography-Q-TOF-MS/MS, Network pharmacology