Abstract: BackgroundThe prenatal stress can cause fetal rats injury,it is closely related to congenital impairment-induced kidney qi damage.Jinkui Shengqi Pills can improve the kidney qi damage of pregnant rats with post traumatic stress disorder（PTSD）,and can improve the pregnant rats’ PTSD-induced abnormal growth of offspring rats.Objective　Jinkui Shengqi Pills were applied in pregnant rats with PTSD to treat fetal rats injury,the gene expression profile and mechanism were explored.Methods　The study was implemented between July 2014 and March 2015,36 initial pregnant SD rats were divided into 3 groups by random number table method:control group（n=12）,PTSD group（n=12）,and Jinkui Shengqi Pill group（n=12）.The PTSD model rats were established during the second trimester（the 7th-13th day after gestation） in PTSD group and Jinkui Shengqi Pill group.The rats in control group and PTSD group were fed with conventional food under normal experiment condition.The rats in Jinkui Shengqi Pill group were fed with food consisting of Jinkui Shengqi Pill until delivery.Gene expression of 30 days old rats was detected by gene expression profile chips.Results　According to the gene expression profile analysis of 30 days old rats in PTSD group and blank group,there were 112 differentially expressed genes,including 70 highly expressed genes and 42 low expressed genes,among which 53 genes returned to normal by using Jinkui Shengqi Pills,these 53 genes were mainly involved in endocytosis pathway and MAPK signaling pathway.Conclusion　Applying Jinkui Shengqi Pills in pregnant rats with PTSD can treat fetal rats injury,the underlying molecular biology mechanism is related to the differential expression of 53 genes,these 53 genes were mainly involved in endocytosis pathway and MAPK signaling pathway.

Key words: Jinkui Shengqi pills, Post traumatic stress disorder, Gene expression profiling, MAPK signaling pathway, Endocytosis pathway

摘要： 背景　孕期应激所致胎损与先天受损所致的肾气受损密切相关，而金匮肾气丸补肾后可改善母代孕期创伤后应激障碍综合征（PTSD）所损之肾气，改善母代PTSD导致的子代生长发育异常。目的　研究金匮肾气丸防治PTSD孕鼠所致胎损的基因表达谱机制。方法　2014年7月—2015年3月，将首次受孕的36只SD孕鼠按照随机数字表法分为空白对照组、PTSD组、中药组，每组12只。PTSD组和中药组大鼠于孕中期（妊娠第7~13天）进行PTSD造模。空白组和PTSD组正常实验条件下常规饲料喂养，中药组造模同时予以金匮肾气丸饲料至分娩。采用表达谱芯片检测30日龄子鼠的基因表达情况。结果　PTSD组与空白对照组子鼠基因表达谱分析显示，共有112条基因差异表达，其中高表达70条，低表达42条。金匮肾气丸可使得其中的53条差异表达基因恢复正常，这53条基因主要参与胞吞通路与丝裂原活化蛋白激酶（MAPK）信号通路。结论　金匮肾气丸防治孕鼠PTSD胎损的分子生物学机制与53条基因的差异表达有关，主要涉及胞吞通路与MAPK信号通路。

关键词: 金匮肾气丸, 创伤后应激障碍综合征, 基因表达谱, MAPK信号通路, 细胞内吞