Abstract: Objective　To explore the difference of expression levels of ApoAⅠ,ApoAⅡ,ApoB and AZGP1 among patients with lung spleen qi deficiency syndrome and HIV/AIDS,and provide the basis for research of TCM syndrome features of HIV/AIDS.Methods　From December 2013 to June 2014,28 HIV/AIDS patients with lung spleen qi deficiency syndrome from a certain area of He’nan province were selected as HIV/AIDS with lung spleen qi deficiency syndrome group （FP group）.In the same period,16 healthy people from the same region were selected as control group （JK group）.Serum of objects was collected,and the levels of　CD4,CD8 and CD4/CD8 were detected.The levels of ApoAⅠ,ApoAⅡ,ApoB,AZGP1 were identified by iTRAQ-MS technology,and detected by ELISA method.Results　The level of CD4 and CD4/CD8 in FP group were significantly lower than those in JK group （P<0.05）.There was no significant difference in CD8 level between the two groups （P>0.05）.There was no significant difference in ApoAⅠ level identified by iTRAQ-MS technology between the two groups （P>0.05）.The ApoAⅡ level identified by iTRAQ-MS technology in FP group was higher than that in JK group （P<0.05）.Levels of ApoB and AZGP1 identified by iTRAQ-MS technology in FP group were lower than those in JK group （P<0.05）.There was no significant difference in ApoAⅠ level detected by ELISA method between the two groups （P>0.05）.The ApoAⅡ level detected by ELISA method in FP group was higher than that in JK group （P<0.05）.Levels of ApoB and AZGP1 detected by ELISA method in FP group were lower than those in JK group （P<0.05）.Conclusion　ApoAⅡ level increased,levels of ApoB and AZGP1 decreased in serum of HIV/AIDS patients with lung spleen qi deficiency syndrome,and this is related to lipid metabolism.

Key words: HIV/AIDS, Lung spleen qi deficiency syndrome, Apolipoprotein

摘要： 目的　探讨人类免疫缺陷病毒（HIV）/获得性免疫缺陷综合征（AIDS）肺脾气虚证患者载脂蛋白（Apo）AⅠ、ApoAⅡ、ApoB、锌α2-糖蛋白（AZGP1）表达水平的差异，为研究HIV/AIDS中医证候提供依据。方法　2013年12月—2014年6月选取河南省某地区的HIV/AIDS肺脾气虚证患者28例为HIV/AIDS肺脾气虚证组（FP组）。同期选取同地区健康人16例为对照组（JK组）。采集研究对象血清，检测CD4、CD8水平及CD4/CD8，采用iTRAQ-MS技术鉴定ApoAⅠ、ApoAⅡ、ApoB、AZGP1水平，酶联免疫吸附试验（ELISA）法检测ApoAⅠ、ApoAⅡ、ApoB、AZGP1水平。结果　FP组CD4水平低于JK组，CD4/CD8小于JK组（P<0.05）；两组CD8水平比较，差异无统计学意义（P>0.05）。两组iTRAQ-MS技术鉴定ApoAⅠ水平比较，差异无统计学意义（P>0.05）；FP组iTRAQ-MS技术鉴定ApoAⅡ水平高于JK组，iTRAQ-MS技术鉴定ApoB、AZGP1水平低于JK组（P<0.05）。两组ELISA法检测ApoAⅠ水平比较，差异无统计学意义（P>0.05）；FP组ELISA法检测ApoAⅡ水平高于JK组，ELISA法检测ApoB、AZGP1水平低于JK组（P<0.05）。结论　HIV/AIDS肺脾气虚证患者血清中ApoAⅡ水平升高，ApoB、AZGP1水平降低，与脂质代谢有关。

关键词: 艾滋病, 肺脾气虚证, 载脂蛋白