关键词: 百日咳, 百日咳鲍特菌, 实时聚合酶链反应, 细菌学技术, 灵敏度, 特异度

Abstract: Background　The event caused by insufficient potency of pertussis vaccine drawed great public concern．Owing to the limitations of pertussis laboratory test，it is hard to meet the clinical needs of rapid etiological diagnosis．Objective　To evaluate the effect of a commercialized quantitative polymerase chain reaction（Q-PCR）kit on diagnosis of infections caused by bordetella pertussis（BP）．Methods　A total of 302 nasopharyngeal swabs of children with cough but no heat and 50 nasopharyngeal swabs of children clinically diagnosed with pertussis were collected in Beijing Children's Hospital ，Capital Medical University from November 2016 to April 2017.Q-PCR and classical bacterial culture method were used to detect whether 302 children with cough but no heat were infected by BP as well as the corresponding．Positive rates of BP in 50 clinically diagnosed cases by two methods were compared to comprehensively evaluate the clinical value of Q-PCR．Results　Compared with culture method，the sensitivity of Q-PCR in 302 cases was 78.6%（11/14），the specificity 95.8%（276/288），the positive predictive value 47.8%（11/23），and the negative predictive value 98.9%（276/279）．The positive rate detected by Q-PCR was 34.0% (17/50) and that by bacterial culture was 22.0% (11/50). There was no significant difference between the two methods（χ2=3.125，P=0.077）．Conclusion　Compared with culture method，the Q-PCR detection method achieved a higher sensitivity and specificity．Two methods had a similar positive rate in providing etiological diagnosis for patients clinically diagnosed with pertussis.

Key words: Pertussis, Bordetella pertussis, Real-time polymerase chain reaction, Bacteriological techniques, Sensitivity, Specificity