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Table of Content

    15 December 2025, Volume 28 Issue 35
    World General Practice/Family Medicine
    Contents of Royal Australian College of General Practitioners Guidelines for Preventive Activities in General Practice, 10th Edition
    2025, 28(35):  0-C2.  DOI: 10.12114/j.issn.1007-9572.2025.35.101
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    Guidelines·Consensus
    Expert Consensus on the Application of Virtual Reality Technology in Home-based Rehabilitation for Cancer-related Insomnia (2025 Edition)
    Community Rehabilitation Working Committee of Chinese Rehabilitation Medicine Association
    2025, 28(35):  4385-4396.  DOI: 10.12114/j.issn.1007-9572.2025.0227
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    To thoroughly implement the concept of whole-cycle rehabilitation and further standardize the application and management of virtual reality (VR) technology in home-based rehabilitation for patients with cancer-related insomnia (CRI), the Community Rehabilitation Working Committee of Chinese Rehabilitation Medicine Association, in collaboration with experts from multidisciplinary fields including oncology, psychology, rehabilitation medicine, nursing, public health, and digital medical technology, has jointly formulated Expert Consensus on the Application of Virtual Reality Technology in Home-based Rehabilitation for Cancer-related Insomnia (2025 Edition) based on evidence-based medicine principles and domestic and international clinical practice experiences. This consensus provided standardized guidance on the mechanism, intervention protocols, implementation process, efficacy evaluation indicators, and safety assessments of VR technology in home-based CRI rehabilitation. It aimed to establish a set of scientific and standardized process framework and a variety of options. Through the implementation of this consensus, it ensured that VR technology was applied reasonably and effectively in home-based rehabilitation for CRI patients, enhancing the scientific nature and safety of clinical practice, promoting the standardized development of digital technologies in the field of cancer rehabilitation, facilitating patients' achievement of whole-cycle rehabilitation, and improving their quality of life.

    Hot Topics in General Practice
    Diagnostic Strategies for Medically Unexplained Symptoms
    LIU Ruihong, CHEN Kai, LI Zhuo, SUN Zuoya, CHEN Zhiyuan
    2025, 28(35):  4397-4402.  DOI: 10.12114/j.issn.1007-9572.2023.0434
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    Medically unexplained symptoms (MUS) is a common problem in primary medical care. The etiology of MUS is unclear, which brings continuous pain to patients and affects the doctor-patient relationship. As a result, the management of MUS is a challenge for general practitioners. Combined with relevant literature and guidelines, this paper expounds the diagnosis and treatment ideas of MUS, so as to help general practitioners identify and manage MUS in clinical practice effectively, and finally achieve the purpose of improving the treatment effect of patients and building a harmonious doctor-patient relationship.

    Analysis of the Thematic Structure and Differences in Doctor-patient Conversations in General Practice Clinics
    GONG Zhexi, MA Li, ZHOU Jiaxin, CUI Puan, LIN Menglan, HE Zhong
    2025, 28(35):  4403-4408.  DOI: 10.12114/j.issn.1007-9572.2024.0594
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    Background

    The doctor-patient relationship is the focus of attention in the medical field. General practice clinic is an important place for providing continuous and comprehensive medical services, and the communication ability of general practitioners (GPs) is particularly important for improving the doctor-patient relationship. However, there are few studies on the current situation of doctor-patient communication in general practice clinic in China.

    Objective

    To understand the characteristics and current situation of doctor-patient conversation in general practice clinic, in order to provide empirical support for improving communication skills of GPs, optimizing doctor-patient communication strategies, and building a harmonious doctor-patient relationship.

    Methods

    Convenience sampling method was used to select eight GPs from one tertiary hospital and three community health service centers (stations). Natural observation method was used to collect 100 doctor-patient conversation corpus from May to August 2024. The Roter Interaction Analysis System (RIAS) was used to classify, code and quantify the doctor-patient conversation, describe the topic structure of the conversation, and analyze the discourse differences.

    Results

    A total of 18 667 doctor-patient utterances were collected; 8 784 from doctors and 9 883 from patients.The verbal dominance ratio was 0.89, the patient-centeredness score was 1.27, and the psycho-biomedical ratio was 0.10 in the general practice clinic. The three most frequent topics of physician discourse were biomedical information giving (28.78%), active dialogue (25.18%) and biomedical information seeking (13.79%), while the three most frequent topics of patient discourse were biomedical information giving (34.68%), active dialogue (28.45%) and social language (14.82%). GPs were more likely to ask for biomedical information (P=0.001) and psycho-social information (P=0.003) than patients, but less likely to give biomedical information (P<0.05) and psycho-social information (P<0.001) than patients. Patients had more social language than doctors (P=0.003). The verbal dominance ratio, biomedical information seeking, biomedical information giving, biomedical advice and guidance, and step language of GPs in tertiary hospitals were higher than those in community GPs (P<0.05), while the psycho-biomedical ratio, psycho-social advice and guidance, and social language of GPs in tertiary hospitals were lower than those in community GPs (P<0.05). The frequency of biomedical information seeking, biomedical information giving and positive dialogue of GP-patient in the tertiary hospitals were higher than those in the community GP-patient (P<0.05), while the social language of GP-patient in the community was significantly higher than that in the tertiary hospitals (P<0.01) .

    Conclusion

    The patient occupies a dominant position in the discourse of the doctor-patient conversation in the general practice clinic. The theme of the doctor-patient conversation is mainly biomedical, and the degree of "patient-centered" is relatively good. Compared with patients, general practitioners had more "seeking discourses" and less "giving discourses". Compared with the GPs in the community, the GPs in the tertiary hospitals have a higher proportion of language dominance, more biomedical discourse, and less psycho-social discourse. GPs should pay more attention to patients' emotional needs, information acquisition needs and psycho-social factors of the disease, so as to improve doctor-patient communication skills and medical service quality.

    Original Research
    The Correlation between Heart Rate Variability and Cerebral Infarction in Patients with Type 2 Diabetes Mellitus
    XU Jun, ZHANG Jie
    2025, 28(35):  4409-4413.  DOI: 10.12114/j.issn.1007-9572.2025.0091
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    Background

    Heart rate variability (HRV) is associated with cerebral infarction, although this association has been less studied in patients with type 2 diabetes mellitus (T2DM) .

    Objective

    To explore the correlation between HRV and cerebral infarction in T2DM patients.

    Methods

    A total of 577 T2DM patients hospitalized in Ningbo No.2 Hospital between January 2020 and August 2022 were selected. According to the presence or absence of cerebral infarction, patients were divided into the cerebral infarction group and non-cerebral infarction group. The general data and laboratory results were collected. All patients received 24-hour Holter monitoring. HRV parameters, including the standard deviation of normal-to-normal intervals (SDNN), the root mean square of successive RR interval differences (rMSSD), and the percent of adjacent RR intervals with a difference more than 50 ms (PNN50) were recorded. Multivariate Logistic regression analysis was used to analyze the correlation between HRV and cerebral infarction. Patients were further divided into the decreased SDNN group (SDNN<100 ms) and normal SDNN group (SDNN≥100 ms) based on the SDNN measurements in HRV, and the correlation with cerebral infarction was explored.

    Results

    A total of 577 T2DM patients were enrolled, including 287 cases in the cerebral infarction group and 290 cases in non-cerebral infarction group. Multivariate Logistic regression analysis showed that SDNN was the independent influencing factor for cerebral infarction in T2DM patients (OR=0.970, 95%CI=0.961-0.979, P<0.05). There were 264 cases in the decreased SDNN group and 313 cases in the normal SDNN group. Multivariate logistic regression showed a significantly higher risk of cerebral infarction in the decreased SDNN group than the normal SDNN group (OR=4.164, 95%CI=2.769-6.262, P<0.05) .

    Conclusion

    The reduction of HRV parameters in T2DM patients is closely correlated to cerebral infarction. With the reduction of SDNN, the risk of cerebral infarction in patients increases.

    Effect of Type 2 Diabetes Mellitus on the Efficacy of Nucleoside (Acid) Analogues in the Treatment of Chronic Hepatitis B
    ZENG Minghui, KUAI Wentao, CHEN Lin, HAN Jiaxin, XU Lianxin, GE Liying, DAI Rongrong, MI Yuqiang, XU Liang
    2025, 28(35):  4414-4420.  DOI: 10.12114/j.issn.1007-9572.2024.0563
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    Background

    Nucleoside (acid) analogues (NAs) have become the primary treatment for chronic hepatitis B (CHB), but the impact of type 2 diabetes (T2DM) on the efficacy was unclear.

    Objective

    To identify the effect of T2DM on the efficacy of NAs antiviral therapy in CHB patients.

    Methods

    Patients with CHB who underwent liver biopsy in Tianjin Second People's Hospital from January 2015 to June 2023 and newly treated with NAs were included (n=350) .The patients were divided into T2DM-CHB group and CHB group according to their medical history. After matching according to gender, age, HBV DNA level, HBeAg status, ALT and AST level, 238 patients were finally included (T2DM-CHB group: n=70; CHB group: n=168). The patient's medication status, as well as liver and renal function, virological indicators and other data were reviewed once every 6 months for a total of 5 years. To evaluate the effectiveness of early treatment of NAs, follow-up was conducted once at the first and third months after the initiation of antiviral therapy.

    Results

    After receiving NAs treatment, the serum lgHBV DNA level in T2DM-CHB group was significantly higher than that in CHB group at 1, 3, 6 and 18 months (P<0.05). The HBsAg value of T2DM-CHB group was significantly higher than that of CHB group at 12, 30 and 36 months (P<0.05). Kaplan-Meier survival curve showed that there was a significant difference between the two groups in the time to achieve complete virological response (CVR) for the first time (χ2=14.144, P<0.001), and the median time of first CVR in T2DM-CHB group and CHB group was 18.9 months and 14.3 months respectively. The cumulative clearance rate and seroconversion rate of HBeAg in T2DM-CHB group were lower than those in CHB group at 6, 12, 18, 24 and 30 months after treatment (P<0.05). In terms of liver function recovery, the accumulation rate of normal liver function in T2DM-CHB group was also lower than that in CHB group at 1, 3, 6, 12, 18 and 24 months after treatment (P<0.001). The results of multivariate Cox regression showed that T2DM (HR=0.706, 95%CI=0.584-0.854), HBV DNA (HR=0.624, 95%CI=0.534-0.730), eGFR (HR=1.197, 95%CI=1.017-1.409) were the factors influencing the time of the first CVR in patients with CHB (P<0.05) .

    Conclusion

    The combination of T2DM weakened the virological response of CHB patients to NAs, and prolonged the time to achieve CVR and liver function recovery.

    Predictive Value of Triglyceride-glucose Index for Prognosis in Patients with Pulmonary Embolism
    MI Lingyu, CHEN Lianxin, GAO Hengbo, WANG Bincheng
    2025, 28(35):  4421-4429.  DOI: 10.12114/j.issn.1007-9572.2024.0663
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    Background

    The triglyceride-glucose (TyG) index, a surrogate marker of insulin resistance (IR), is associated with the pathogenesis of metabolic syndrome and pulmonary embolism (PE) .

    Objective

    To investigate the association between the TyG index and clinical outcomes in critically ill patients with PE.

    Methods

    This retrospective cohort study utilized data from the Medical Information Mart for Intensive Care Ⅳ (MIMIC-Ⅳ) database. PE patients admitted to the intensive care unit (ICU) between 2008 and 2019 were included. Patients were divided into four groups according to the quartiles of their TyG index on the first day of ICU admission. The primary outcome was all-cause mortality during hospitalization, including in-hospital mortality and ICU mortality. The association between the TyG index and patient prognosis was assessed using Cox proportional hazards regression analysis and restricted cubic splines (RCS) .

    Results

    A total of 391 patients were included in the study, with a mean age of (62.2±16.5) years. The average TyG index of the entire cohort was (4.95±0.41). The observed in-hospital mortality rate was 19.18% (75/391), while the ICU mortality rate was 10.23% (40/391). Based on the TyG index at admission, patients were divided into four quartile groups (TyG index: Q1: 3.81-4.68, Q2: 4.69-4.90, Q3: 4.91-5.18, and Q4: 5.19-7.05), with 98, 97, 98, and 98 patients in each group, respectively. After adjusting for covariates, Cox proportional hazards regression analysis showed that an increasing TyG index was associated with a higher risk of in-hospital mortality (HR=4.14, 95%CI=1.40-12.28, P=0.010) and ICU mortality (HR=6.45, 95%CI=1.27-32.60, P=0.024). Compared to Q1, Q4 TyG quartile had a significantly increased risk of in-hospital mortality (HR=4.17, 95%CI=1.33-13.03, P=0.014). RCS analysis indicated a linear increase in both in-hospital and ICU mortality rates with higher TyG index levels (Pfor nonlinearity=0.833 and 0.669, respectively) .

    Conclusion

    There is a significant association between the TyG index and all-cause mortality in critically ill PE patients, suggesting that the TyG index may serve as a valuable predictor of in-hospital and ICU mortality risk.

    Alterations in Serum RAGE/NF-κB Pathway Expression Levels and Their Association with Cognitive Function in Wilson's Disease Patients with Mild Cognitive Impairment
    NI Mingzhu, WANG Li, XU Zhenjing, SHI Qiao, HOU Zhifeng
    2025, 28(35):  4430-4434.  DOI: 10.12114/j.issn.1007-9572.2025.0072
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    Background

    Wilson's disease (WD) is associated with elevated inflammatory levels and closely linked to cognitive impairment. Studies suggest that activation of the receptor for advanced glycation end products (RAGE) /nuclear factor kappa B (NF-κB) signaling pathway is a critical inflammatory mechanism mediating cognitive dysfunction, yet expression of key molecule in serum of WD patients with mild cognitive impairment (MCI) remains unclear.

    Objective

    To investigate changes in serum RAGE/NF-κB pathway and their impact on MCI in WD patients.

    Methods

    A total of 23 WD patients with MCI (WD-MCI group) hospitalized at the Encephalopathy Center, First Affiliated Hospital of Anhui University of Chinese Medicine from January 2024 to January 2025 were enrolled. Age-, sex-, and education-matched healthy controls (n=23) were recruited. Cognitive function was assessed using the Mini-mental State Examination (MMSE), Hopkins Verbal Learning Test (HVLT), Boston Naming Test-Second Edition (BNT-2), Clock Drawing Test (CDT), Trail Making Test-A (TMT-A), and Instrumental Activities of Daily Living Scale (IADL). Serum RAGE levels and NF-κB p65 mRNA expression were measured using enzyme-linked immunosorbent assay (ELISA) and RT-qPCR, respectively. Differences in cognitive scores, serum RAGE levels, and NF-κB p65 mRNA expression between groups were compared. Pearson or Spearman correlation analysis evaluated associations between RAGE/NF-κB p65 mRNA levels and cognitive scores.

    Results

    Compared with controls, the WD-MCI group showed significantly lower MMSE, HVLT, BNT-2, and CDT scores, and higher TMT-A and IADL scores (P<0.05). Serum RAGE levels and NF-κB p65 mRNA expression were elevated in the WD-MCI group (P<0.05). Correlation analysis revealed that serum RAGE levels and NF-κB p65 mRNA expression negatively correlated with MMSE (r=-0.866, -0.729), HVLT (r=-0.721, -0.728), BNT-2 (rs=-0.381, -0.382), and CDT (rs=-0.788, -0.709) scores, and positively correlated with TMT-A (r=0.774, 0.524) and IADL (rs=0.433, 0.376) scores (P<0.05) .

    Conclusion

    WD patients exhibit increased serum RAGE/NF-κB pathway activity, which is significantly associated with mild cognitive impairment.

    Investigation on the Current Application of Injectable Treatment for Hemorrhoids in China
    HUO Xingxiao, SUN Songpeng, LONG Junhong, LIANG Longyu, CHU Hongchuan, ZHOU Yangyang, LIU Yan, LIU Jiaxin
    2025, 28(35):  4435-4441.  DOI: 10.12114/j.issn.1007-9572.2025.0078
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    Background

    Hemorrhoids are one of the common diseases in anus and intestines, and injection therapy is a commonly used treatment, but there is a lack of national data on the depth and standardized application of injection therapy, so it is necessary to carry out this research study.

    Objective

    To investigate the depth and breadth, standardized application, effectiveness and safety of hemorrhoidal injection therapy and the reasons for its failure to be carried out in China.

    Methods

    An online questionnaire was issued to the members of the Professional Committee of Anal and Intestinal Diseases of the Chinese Association for Medical Education from July to November 2022, and the content of the questionnaire was designed by ourselves, with a total of 37 questions, including demographic information, methods of treating hemorrhoids, the application of injectable treatments, and complications, etc. The data were statistically analyzed using SPSS 21.0 software.

    Results

    A total of 335 questionnaires were recovered and 312 questionnaires were valid (93.1% recovery rate). There were 293 (93.9%) doctors using sclerotherapy, 153 (54.2%) doctors implementing more than 500 cases of injection therapy, and the difference was not statistically significant when comparing the number of injection therapy carried out by anorectal doctors of different hospital levels, hospital natures, and types of hospitals (P>0.05). 293 (93.9%) and 263 (84.3%) doctors believed that injection therapy was suitable for the treatment of internal hemorrhoids and the internal portion of mixed hemorrhoids. For internal hemorrhoids of degree Ⅰ and Ⅱ, the percentage of sclerotherapy injection was 55.4% (173/312) and 79.2% (247/312), respectively. For the choice of anesthesia, local anesthesia was predominant in 229 (73.4%) and lumbar anesthesia in 145 (46.5%) cases. Sclerotherapy was applied in 280 (89.7%), accounting for the first use of sclerotherapy; 201 (64.4%) chose to treat three hemorrhoids in a single treatment, and for hemorrhoids in the anterior part of the anus, 140 (44.9%) of the physicians thought that they could be injected, while 172 (55.1%) thought that sclerotherapy should not be injected. Dosage analysis showed that anti-hemorrhoidal sclerotherapy single site injections were concentrated at 1-5 mL in 80.7% (92/114) and low-dose (0-3 mL) regimens in 56.7% (59/104) of cases; despite the fact that 233 (74.7%) endorsed the curative properties of injections alone, 227 (88.8%) of the clinical practices combined the procedures, with hemorrhoidectomies accounting for 64.7% (202/312) of the cases. The 1-year recurrence rate of <10% assessed for injection alone amounted to 90.3% (233/258), but decreased to 58.9% (146/248) at the 5-year assessment; the complication rate of patients treated with injections showed anal pain in 134 (42.9%), urinary retention in 90 (28.8%), and hardness at the injection site in 182 (58.3%), which were the common complications. Other serious complications included perianal abscess (5.1%, 16 cases), sepsis (1.3%, 4 cases) and rectal perforation (1.6%, 5 cases). The main reasons for not carrying out injection therapy were cited by 15 (35.7%) as hospital management factors, 9 (21.4%) as price, 8 (19.0%) as questionable efficacy, and 6 (14.3%) as drug shortage.

    Conclusion

    Hemorrhoid injection therapy has been widely recognized and applied by anorectal physicians in China, with 93.9% of physicians carrying out this therapy, more than 54.2% operating more than 500 cases, and the popularity of this therapy did not differ significantly among all levels and types of hospitals. At least 55.4% of doctors can standardize the indications (Ⅰ-Ⅱ degree internal hemorrhoids are more inclined to use injection treatment). Anti-hemorrhoidal spirit is the most commonly used sclerosing agent, and more than 3 hemorrhoid nuclei are injected at one time. Hemorrhoidal injection therapy is efficacious, with 90% of physicians reporting a 1-year recurrence rate of <10%, and long-term recurrence rate data supporting its reliability. Nearly 90% of physicians preferred to combine with other procedures, reflecting a lack of confidence in injections alone and the need to clarify the "symptomatic relief" goal of device therapy and improve patient communication. Hemorrhoidal injection therapy as a safe and effective hemorrhoidal management tool is suitable for national promotion, but needs to strengthen the operation standardization training and complication prevention and control system construction.

    Correlation between Dietary Inflammatory Index and Delayed Onset of Lactogenesis in Women of the Third Trimester
    CHEN Huiyu, XU Ziyan, LI Yuhong
    2025, 28(35):  4442-4448.  DOI: 10.12114/j.issn.1007-9572.2024.0679
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    Background

    Delayed onset of lactogenesis (DOL) is an important cause for reduced exclusive breastfeeding rates. The dietary inflammatory index (DII) has been shown to be associated with a variety of adverse health outcomes, but the relationship with DOL is not well understood.

    Objective

    Understanding DII levels of women in the third trimester and exploring the impact on DOL.

    Methods

    A convenience sampling method was used to select women in the third trimester who underwent routine obstetric examinations in the First Affiliated Hospital of Anhui Medical University from December 2023 to June 2024 as the study subjects and were followed up until 72 hours postpartum, and the maternal self-perceived breast distension method was used to determine whether they were in the DOL population. General demographic characteristics and information related to pregnancy and childbirth were collected using a general information questionnaire, a food frequency questionnaire (FFQ) was used to collect information on dietary intake and calculates the DII, and the delivery and postpartum data questionnaire were used to collect relevant information about labor and postpartum. Exploring the relationship between DII and DOL using binary Logistic regression analysis and stratified the factors of age, preconception BMI, and presence of gestational diabetes mellitus.

    Results

    A total of 228 questionnaires were distributed and 217 questionnaires were validly returned, with an effective recovery rate of 95.2%. Among them, 68 (31.3%) with DOL. The DOL and the non-DOL mothers were statistically different in terms of the number of pregnancies, the quality of sleep, gestational weight gain, and the presence of mother-infant separation after delivery (P<0.05). The pregnant women in the high DII group had a higher incidence of DOL than those in the middle and low DII groups according to the three quartiles of DII scores from smallest to largest (P<0.017). The results of binary Logistic regression analysis showed that the risk of DOL was 2.817 times higher in the high DII group than in the low DII group (95%CI=1.244-6.381, P=0.013). In the third trimester group of women aged<35 years with preconception BMI<24.0 kg/m2, the risk of DOL in women with high DII levels was 3.144 times (95%CI=1.346-7.344, P<0.05) and 2.666 times (95%CI=1.072-6.630, P<0.05) higher than in women with low DII levels, respectively.

    Conclusion

    There was a correlation between DII and DOL, with higher DII increasing the risk of DOL, especially in the group of women in late pregnancy who are <35 years of age and with preconception BMI <24.0 kg/m2. Maternal and child health care providers should provide individualized dietary guidance to pregnant women, increase the intake of anti-inflammatory diets to reduce the incidence of DOL and increase the rate of exclusive breastfeeding.

    Gas6 Expression in Breast Cancer and Correlation with Clinical Pathological Features and Prognosis
    ZHOU Qianmei, MIAO Kali, ZHONG Yongming, ZHANG Fuman, ZHANG Zhe, LI Chengyi
    2025, 28(35):  4449-4456.  DOI: 10.12114/j.issn.1007-9572.2024.0692
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    Background

    Targeted drugs for the AXL/Gas6 signaling pathway have become a new focus in current cancer drug research. However, there is significant controversy over the expression and role of Gas6 in breast cancer.

    Objective

    To investigate the expression of Gas6 and AXL in breast cancer and to explore the correlation of GAS6 with clinicopathological features and prognosis.

    Methods

    A total of 60 patients with invasive breast cancer who were treated at the Department of Breast Surgery, Fujian Medical University Affiliated Mindong Hospital from October 2019 to February 2022 were included. Surgical resection specimens of cancerous and adjacent tissues were collected from these patients. The expressions of Gas6 and AXL were detected using immunohistochemical staining and real-time fluorescence quantitative PCR (RT-qPCR). Spearman rank correlation analysis was employed to assess the correlation between the two factors and the relationship between Gas6 and clinical pathological features. Additionally, gene expression data for Gas6 and AXL in breast cancer and normal breast tissues were collected from the GEPIA2.0, UALCAN, and METABRIC databases for supplementary validation. Survival data for different breast cancer subtypes were collected from the METABRIC database and Kaplan-Meier Plotter data platform. The survival curve was plotted using the Kaplan-Meier method, and the Cox proportional hazards model was used to evaluate the effect of Gas6 expression level on overall survival (OS) and recurrence-free survival (RFS) .

    Results

    Immunohistochemical staining and RT-qPCR detection results showed that the relative expression levels of Gas6 protein and mRNA in breast cancer tissues were lower than those in adjacent non-cancerous tissues (P<0.001). In contrast, the relative expression levels of AXL protein and mRNA in breast cancer tissues were higher than those in adjacent non-cancerous tissues (P<0.001). Spearman correlation analysis based on the databases revealed that there was a positive correlation between Gas6 and AXL mRNA expression in the total breast cancer population and four subtypes of patients (rs=0.480, P<0.001). Moreover, statistically significant differences were observed in Gas6 mRNA expression levels among patients with different breast cancer molecular subtypes, primary tumor stages, histological grades, chemotherapy treatments and regional lymph node stages (P<0.05). Kaplan-Meier survival analysis indicated that in the overall breast cancer population and among patients with Luminal A subtype, the group with high Gas6 mRNA expression in cancerous tissues had a longer OS and RFS compared to the low expression group. Additionally, for Luminal B subtype patients, RFS of the high Gas6 mRNA expression group in cancerous tissues is also longer than that of the low expression group, with these differences being statistically significant (P<0.05) .

    Conclusion

    High expression of tumor cell-derived Gas6 is a favorable prognostic factor for invasive breast cancer. Targeting Gas6 and its receptor in breast cancer represents a promising therapeutic approach, particularly when combined with immunotherapy.

    Predictive Value of Convolutional Neural Network for Chronic Kidney Disease Progression Based on Chronic Kidney Disease Dataset
    SONG Xinyuan, CHANG Wenxiu, ZHANG Wenyu, YANG Tingting, WANG Kai
    2025, 28(35):  4457-4463.  DOI: 10.12114/j.issn.1007-9572.2024.0604
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    Background

    Early and accurate prediction of the risk of developing end-stage renal disease (ESRD) is essential for medical decision-making. In the field of chronic kidney disease (CKD), previous studies have reported the impact of various factors and the percentage decline in estimated glomerular filtration rate (eGFR) in the previous 2 years on the development of ESRD from a medical perspective. Traditional risk assessment methods usually rely on expert experience, simple statistical analyses, and limited biomarkers, which face obvious limitations when dealing with complex, multidimensional health data, whereas the use of machine learning algorithms, such as artificial neural networks, can significantly improve the accuracy, sensitivity, and specificity of risk prediction.

    Objective

    Based on multiple algorithms, this study explored the predictive value of 2-year mean levels of clinical parameters and the rate of change of eGFR over a period of 2 years in the progression of CKD to ESRD.

    Methods

    The dataset for this study was obtained from a retrospective cohort of the Japanese CKD population at Teikyo University Hospital, Japan, from 2008 to 2014, 700 patients were enrolled in the study cohort. Two datasets were obtained based on this cohort, a baseline dataset and a 2-year time-averaged dataset. Logistic regression (LR), multilayer perceptual machine (MLP), support vector machine (SVM), extreme gradient boosting tree (XGBoost), and two-dimensional convolutional neural network (CNN) algorithms were used to predict whether a patient would reach ESRD after several years and to derive probabilities. The dataset is balanced at both the data and algorithmic levels, and medical significance is demonstrated using comparative trials.

    Results

    Using LR, MLP, SVM, and XGBoost as the baseline models, the comparison experiments showed that the CNN model was the best, with an accuracy of 94.8%, precision of 80.3%, recall of 78.2%, and F1 score of 78.4%. The evaluation metrics of the five models on the two-year time-averaged dataset were significantly higher than those on the baseline dataset, especially the recall rate. In addition, models that included the eGFR decline rate variable over two years outperformed models that did not include this variable. Recall improved considerably after addressing the imbalance in the dataset categories.

    Conclusion

    This study demonstrates that a two-dimensional CNN model based on the CKD dataset can guide healthcare professionals to make better clinical treatment decisions, that the mean level of clinical parameters in the first 2 years and the percentage decline in eGFR over 2 years are significant in predicting dialysis events, and that comprehensive management in the first 2 years is essential to delay the onset of ESRD.

    Study on the Predictive Value of Cystatin A for Osteonecrosis of the Femoral Head
    TIAN Jiaqing, PENG Peng, WEI Yurou, XIAO Fangjun, HE Xianshun, LIN Kun, LU Shun, HE Mincong, LIU Liangyan, WEI Qiushi
    2025, 28(35):  4464-4472.  DOI: 10.12114/j.issn.1007-9572.2024.0299
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    Background

    Osteonecrosis of the femoral head (ONFH) is a clinically refractory orthopedic disease with a relatively high incidence and poor prognosis. Currently, magnetic resonance imaging (MRI) is the "gold standard" for diagnosing ONFH, but its examination process is cumbersome and the cost is high, so it cannot be used as a tool for routine screening. Therefore, it is urgent to develop new molecular biomarkers for the diagnosis of ONFH.

    Objective

    To study the serum cystatin A (CSTA) level in ONFH patients and evaluate its diagnostic value as a biomarker for ONFH.

    Methods

    Twenty-one ONFH patients who visited the Third Affiliated Hospital of Guangzhou University of Chinese Medicine from May to November 2023 were selected as the experimental group, and 33 healthy volunteers were recruited as the control group at the same time. Serum and femoral head tissue samples were collected from the subjects. Among them, the femoral head tissue samples included 17 patients with ONFH from the experimental group and 6 patients who underwent surgery for femoral neck fractures during the same period, who were designated as the femoral neck fracture (FNF) group. The serum CSTA level was detected by ELISA; The bone tissue morphology was observed by HE staining, and the bone microstructure was detected by CT; Trap staining was used to evaluate the osteoclast differentiation ability in different bone tissues; The expression level of CSTA in bone tissues was evaluated by immunohistochemistry, immunofluorescence staining, and Western Blotting; Spearman rank correlation analysis was used to analyze the correlation between CSTA level and ARCO stage; The receiver operating characteristic (ROC) curve was drawn to evaluate the diagnostic value of CSTA.

    Results

    The results of serum CSTA detection showed that the serum CSTA levels of patients with ARCO stageⅡ-Ⅳ in the experimental group were higher than those in the control group (P<0.05). ARCO stage Ⅱ was lower than ARCO stage Ⅲ, and ARCO stage Ⅲ was lower than ARCO stage Ⅳ (P<0.05). The results of HE staining showed that there were empty bone depressions in ARCO stages Ⅱ, Ⅲ and Ⅳ. The litter rate was higher than that of the FNF group, and that of ARCO stage Ⅲ andⅣ was higher than that of ARCO stage Ⅱ (P<0.05) ; CT quantitative analysis results showed that the bone volume fraction (BV/TV), trabecular bone thickness (Tb.Th), and trabecular bone number (Tb.N) in ARCO Ⅱ and Ⅲ stages were lower than those in the FNF group, and the trabecular bone separation (Tb.Sp) was higher than that in the FNF group (P<0.05) ; Trap staining results showed that the area of Trap-positive cell regions in ARCO Ⅱ and Ⅲ groups was higher than that in the FNF group, and ARCO Ⅱ was higher than ARCO Ⅳ (P<0.05) ; The results of CSTA detection in bone tissues showed that the CSTA expression in ARCO Ⅱ, Ⅲ, and Ⅳ stages was higher than that in the FNF group, and ARCO Ⅳ stage was higher than ARCO Ⅱstage (P<0.05) ; The area under the ROC curve (AUC) of serum CSTA level in diagnosing ONFH was 0.939, with a sensitivity of 78.79% and a specificity of 95.24%.

    Conclusion

    The CSTA protein can affect the repair of necrotic bone in the femoral head by regulating the formation of osteoclasts. In patients with osteonecrosis of the femoral head, the increased expression of CSTA is positively correlated with the severity of the disease.

    Original Research·Public Health Research
    Investigation on the Prevention and Control Capacity of Infectious Diseases and Influencing Factors in Primary Medical and Health Service Institutions in Beijing in 2019-2022
    SU Ning, LIU Pingping, GUO Yumeng, LU Mingyue, ZHU Rui, YU Jianping
    2025, 28(35):  4473-4478.  DOI: 10.12114/j.issn.1007-9572.2024.0243
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    Background

    Community health service institutions are the bottom of the infectious disease prevention and control system, and it's crucial to the control of urban epidemic situations. After the COVID-19, the prevention and control capacity of community health service institutions in Beijing has been improved, but there is a lack of evaluation, and the direction of future improvement is not clear.

    Objective

    To evaluate the changes in the infectious disease prevention and control capacity of community health service institutions in Beijing during the COVID-19 period, analyze the influencing factors, and put forward suggestions for improvement measures, so as to provide a scientific basis for further enhancing the prevention and control capacity of infectious diseases at the grass-roots level.

    Methods

    From 2019 to 2022, questionnaires were conducted on 334, 354, 405 and 405 community health service centers and independent community health service stations willing to cooperate with the survey in Beijing. The quantitative scoring method was used to measure the comprehensive capacity of infectious disease prevention and control of various institutions from the sentinel setting (20 points), pathogen detection of common infectious diseases (30 points), and the emergency capacity of responding to infectious diseases and public health emergencies (50 points). Linear mixed model is used to compare the changes in the prevention and control capacity of various institutions from 2019 to 2022, and the factors affecting the improvement of the prevention and control capacity of infectious diseases are analyzed.

    Results

    The median scores of comprehensive capacity for infectious disease prevention and control in 2019-2022 were 50 (43, 60), 63 (43, 70), 70 (50, 80) and 70 (50, 80), respectively. The differences between each year were statistically significant (P<0.05) .Linear mixed model showed that, organization category, whether to set up preventive health care department, whether to have P2 laboratory, the actual number of employees have an impact on the comprehensive ability of infectious disease prevention and control (P<0.05). The comprehensive capacity of infectious disease prevention and control in community health service centers was 18.94 (95%CI=15.73-22.15, P<0.05) higher than that of independent community health service stations, and the comprehensive capacity of the prevention and health care department was 6.50 (95%CI=4.00-9.01, P<0.05) higher than those without, the institution of infectious disease prevention and control with P2 laboratories was 2.59 (95%CI=0.62-4.55, P<0.05) higher than those without, and the capacity for infectious disease prevention and control of institutions with a larger number of on-the-job staff was increased by 0.05 (95%CI=0.03-0.07, P<0.05) .

    Conclusion

    During the period of COVID-19 disease, the prevention and control capacity of the community health service institutions in Beijing has been improved year by year, mainly influenced by factors such as the type of institutions, the setting of prevention and health care departments, the setting of P2 laboratories and the actual number of people on duty. It is suggested to invest continuously, rationally allocate resources, strengthen personnel training, and establish a sound working mechanism.

    Progress and Trends in the Implementation of Essential Public Health Services in Shenzhen, 2017-2022
    ZHANG Siqi, CHEN Xuehui, WANG Yijing, LIU Lu, LI Mengyu, WANG Jingbo, LIU Gang, YOU Lili
    2025, 28(35):  4479-4486.  DOI: 10.12114/j.issn.1007-9572.2023.0785
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    Background

    Since the National Essential Public Health Service Program was launched in 2009, various services have been commonly carried out across the country, and the implementation and progress of the program varies among provinces and cities due to their different levels of economic development. As a national economic center city and a national innovative city, Shenzhen is at the forefront of informatization technology in cities across the country.

    Objective

    To sort out and summarise the implementation progress and trend of essential public health services in Shenzhen 2017-2022, and provide reference for further promoting the high-quality development of essential public health services in Shenzhen.

    Methods

    From July 2022 to April 2023, collect 56 evaluation indicators of essential public health services in Shenzhen from 2017 to 2022, and analyse the trend of each indicator during the six-year period.

    Results

    (1) The actual funding in place for the National Essential Public Health Service Program in Shenzhen had increased from 80.71 yuan in 2017 to 144.86 yuan in 2022, the total number of primary health workers had increased from 9 992 to 13 758, and the proportion of public health physician in the primary medical staff increased from 5.39% to 5.94%. (2) The average annual growth rates of the electronic health record filing rate, the newborn visit rate, the health management rate for children aged 0-6 years, the early pregnancy filing rate, the postnatal visit rate, maternal system management rate, the health management rate for the elderly, the standardised management rate for patients with severe mental disorders, the standardised medication rate for TB patients, and the rate of TCM health management services for the elderly and children aged 0-36 months, were respectively 6.27%, 0.32%, 2.61%, 1.06%, 0.07%, 0.62%, 8.34%, 2.11%, 0.51%, 9.53%, and 5.01%, while the rates of health record utilisation, standardised management of hypertensive patients, and standardised management of diabetic patients decreased from 77.40%, 72.48%, and 72.09% in 2017 to 36.58%, 64.14%, and 66.32% in 2022. (3) The average annual growth rates of health literacy rate, the proportion of people participating in physical exercise, and the rate of exclusive breastfeeding of infants within six months are 23.75%, 15.86%, and 5.35%, and the average annual growth rates of the neonatal mortality rate, the infant mortality rate, and the mortality rate of children under five years of age were -1.71%, -4.25%, and -1.63%. Respectively, the blood pressure control rate of the managed population and the blood glucose control rate of the managed population decreased from 70.84% and 67.41% in 2017 to 69.33% and 60.82% in 2022, and the average annual growth rates of the cesarean section rate, the incidence of birth defects, the low birth weight rate, the low weight rate of children under the age of 5, the prevalence of anaemia in children under the age of 5, and the obesity rate of children under five-years old were 0.74%, 1.87%, 3.97%, 3.85%, 11.03%, and 33.50%. The rate of public knowledge decreased from 86.26% to 68.77%, and the rate of resident satisfaction increased from 88.51% to 91.00% from 2018 to 2022.

    Conclusion

    Shenzhen's essential public health services from 2017 to 2022 have achieved remarkable results: organization and management and financial expenditure have been strengthened, and the human resources continues to grow stronger; all types of populations have wide coverage, and the effect of health education has begun to show. However, the use of health records needs to be strengthened, and there is a need to further promote the "the elderly, the high pressure, and the diabetes" program management quality development, and constantly improve the knowledge rate and sense of access of the residents.

    Original Research·Appropriate Technology Research
    The Application Value of a Novel Cell Collector for the Esophagus in Screening for Esophageal Lesions
    ZHANG Hui, LUO Miaomiao, YU Xiaohang, LIN Xin, YE Lina, TANG Xuan, LI Jiaxue, XIE Xinyu, LAI Li, FENG Yan, LIU Yonghui, CHEN Weigang
    2025, 28(35):  4487-4493.  DOI: 10.12114/j.issn.1007-9572.2025.0175
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    Background

    China has the highest disease burden of esophageal cancer in the world. Early detection of esophageal cancer and precancerous lesions is key to improving patient survival rates and reducing incidence and mortality rates.

    Objective

    To explore the clinical application value of the esophageal novel cell collector in screening esophageal lesions, and to provide a basis for the governmental health departments to formulate more effective cancer prevention and control policies and public health programs.

    Methods

    From January 2024 to January 2025, 261 patients who completed esophageal novel cell collection, endoscopy, and pathological biopsy were conducted at nine hospitals in the Xinjiang region, including the First Affiliated Hospital of Shihezi University, Hongxing Hospital of the 13th Division, Beitun Hospital of the 10th Division, Kashi Campus of the General Hospital of the 3rd Division, Korla Hospital of the 2nd Division, Fourth Division Hospital, Seventh Division Hospital, Xinjiang Uygur Autonomous Region People's Hospital, and Tumushuk City of Third Division General Hospital. Using pathological biopsy as the gold standard, combined with morphological findings under gastroscopy, the subjects were divided into the early esophageal cancer and precancerous lesion group (n=34), the reflux esophagitis group (n=150), and the esophageal mucosa without abnormalities group (control group) (n=77). The diagnostic efficacy of cytological examination using a novel esophageal cell collector was evaluated in different populations.

    Results

    Cytological examination results: 4 cases of cancer cells, 27 cases of high-grade intraepithelial lesions, 12 cases of low-grade intraepithelial lesions, 101 cases of atypical squamous cells, 111 cases of squamous cell hyperplasia/inflammatory cells, and 6 cases with no intraepithelial lesions or malignant cells. Endoscopic and pathological biopsy results: 4 cases of squamous cell carcinoma/adenocarcinoma cells, 19 cases of high-grade intraepithelial neoplasia, 11 cases of low-grade intraepithelial neoplasia, 20 cases of atypical squamous cells, 15 cases of inflammatory cells, 115 cases of regional edema/cell hyperplasia, and 77 cases of normal cells. The area under the receiver operating characteristic (ROC) curve of the novel esophageal cell collector for screening early esophageal cancer and precancerous lesions in the early esophageal cancer and precancerous lesion group and the control group was 0.933, with a sensitivity of 94.12%, a specificity of 67.53%, a positive predictive value of 56.14%, and a negative predictive value of 96.30%, and an overall accuracy of 75.68%. The area under the ROC curve of the novel esophageal cell collector for screening early esophageal cancer and precancerous lesions in the early esophageal cancer and precancerous lesion group and the reflux esophagitis group was 0.902, with a sensitivity of 94.12%, a specificity of 42.00%, a positive predictive value of 26.89%, a negative predictive value of 96.92%, and an overall accuracy of 51.63%. The new esophageal cell collector was highly safe (with a severe adverse event rate of 0) and highly acceptable (with an average visual analog scale score of 1.3). 93.9% (245/261) of participants indicated their willingness to undergo follow-up examinations for esophageal lesions using this method.

    Conclusion

    The novel esophageal cell collector demonstrates good diagnostic efficacy for early esophageal cancer and precancerous lesions, reducing the need for unnecessary endoscopic examinations. It features simple operation, high efficiency, safety, and high patient acceptability.

    Application of a Standard Diffusion Simulator in Quality Evaluation of DLCO Instruments
    WU Zhongping, HUANG Ruibo, LIN Kuiqing, YU Xinxin, ZHONG Liping, CHEN Shubing, ZHENG Jinping, GAO Yi
    2025, 28(35):  4494-4500.  DOI: 10.12114/j.issn.1007-9572.2025.0008
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    Background

    The accuracy of diffusing capacity of the lung for carbon monoxide (DLCO) instruments directly influences clinical decision-making in pulmonary diseases. However, long-term use of these instruments may lead to performance drift and measurement errors. At present, daily calibration using 3-L syringes and biological controls is commonly applied in clinical practice, but their sensitivity is insufficient to detect potential errors. Therefore, there is an urgent need to explore more precise and objective methods for quality evaluation of these instruments.

    Objective

    To evaluate the accuracy of DLCO instruments using a standard diffusion simulator that mimics human single-breath maneuvers, to identify and correct the sources of measurement errors, to observe the stability of calibration over time, and to assess the application value of this method in combination with biological control tests.

    Methods

    From March to July 2021, four DLCO instruments were tested in the pulmonary function laboratory of the National Clinical Research Center for Respiratory Diseases. A standard diffusion simulator (Hans Rudolph series 5560, USA) was employed with three carbon monoxide concentrations (0.08%, 0.10%, 0.13%) and three inhalation volumes (1.5 L, 3 L, 4.5 L) in different combinations to simulate single-breath DLCO tests. An absolute error of <2 mL·min-1·mmHg-1 between the measured and target DLCO values was defined as the acceptable range. The accuracy of four DLCO instruments (two brands, two models each) was evaluated as baseline, and the sources of measurement errors were analyzed. Each instrument was then corrected according to its error sources, and changes in accuracy before and after calibration as well as within three months were compared. Biological control testing was also performed to observe inter-instrument differences.

    Results

    At baseline, 50% (2/4) of the instruments had a mean absolute DLCO error greater than 2 mL·min-1·mmHg-1. The error sources varied, including damaged components, incorrect calibration methods, and syringe leakage. After targeted corrections, all instruments achieved a mean absolute DLCO error within 2 mL·min-1·mmHg-1 (P<0.001), and this accuracy was maintained for three months. Post-calibration, the differences among instruments in biological control testing were significantly reduced, particularly in the range and coefficient of variation (CV) of DLCO measurements, which showed statistically significant improvements (P<0.05) .

    Conclusion

    DLCO instruments that have been in long-term clinical use show considerable variability in accuracy, with large measurement differences across instruments. Quality evaluation using a standard diffusion simulator is effective and feasible, enabling objective assessment of instrument accuracy and compensating for the limitations of routine calibration. Regular use of simulators is recommended for instrument quality evaluation and quality control.

    Review & Perspectives
    Research Progress of Lactylation in Age-related Diseases
    WU Miaomiao, LI Pengfei, XU Linhui, KANG Lihua, JI Min, WANG Yong, GUAN Huaijin
    2025, 28(35):  4501-4510.  DOI: 10.12114/j.issn.1007-9572.2024.0392
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    Lactylation (Kla) is a novel mechanism of protein post-translational modification, which is formed by covalent coupling of lactate produced by glycolysis with lysine residues, mainly including histone lactylation and non-histone lactylation. At present, the study found that the main through regulating gene transcription and protein expression and subcellular localization to participate in the process of life activity in the cell. With the increasing of age, lactate metabolism in multiple organs of the body was disordered, and recent studies found that lactylation mediated by it could participate in the occurrence and development of a variety of age-related diseases. Therefore, this paper aimed to review the functional and mechanistic studies of lactylation in age-related diseases.

    Research Progress on Drug-related Rapid Eye Movement Sleep Behavior Disorder
    WANG Yi, CHENG Jinxiang, ZHAO Xianchao, REN Jiafeng, ZHANG Liping, DONG Menglong, LIN Zhifeng, SU Changjun
    2025, 28(35):  4511-4516.  DOI: 10.12114/j.issn.1007-9572.2024.0347
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    Rapid eye movement sleep behavior disorder (RBD) is a parasomnia characterized by the loss of muscle atonia and the enactment of dreams during REM sleep, which can significantly impact the quality of life for both patients and their bed partners. RBD can be classified into two types based on the presence or absence of clear precipitating factors: idiopathic rapid eye movement sleep behavior disorder (iRBD) and secondary rapid eye movement sleep behavior disorder (sRBD). sRBD is often associated with conditions such as narcolepsy, autoimmune and inflammatory diseases, and medication use. In recent years, there have been reports of drug-related RBD, particularly associated with antidepressants, antipsychotics, and other medications. This article provided a systematic review of drug-related RBD, summarized its pathological mechanisms and treatment approaches.