Single Nucleotide Polymorphisms in the Mitochondrial Displacement Loop and Age at Onset of Gastric Cancer

doi:10.12114/j.issn.1007-9572.2022.02.003

Chinese General Practice ›› 2022, Vol. 25 ›› Issue (15): 1883-1887.DOI: 10.12114/j.issn.1007-9572.2022.02.003

Special Issue: 肿瘤最新文章合辑；消化系统疾病最新文章合辑

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Single Nucleotide Polymorphisms in the Mitochondrial Displacement Loop and Age at Onset of Gastric Cancer

1. Department of Gastroenterology, the Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, China
2. Office of Educational Administration, the Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, China
3. Department of Rheumatology and Immunology, the Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, China

Received:2021-11-08 Revised:2022-01-03 Published:2022-01-20 Online:2022-04-07
Contact: Ruixing ZHANG
About author:
WANG Y N, WU C S, ZHAO Y, et al. Single nucleotide polymorphisms in the mitochondrial displacement loop and age at onset of gastric cancer[J]. Chinese General Practice, 2022, 25 (15) : 1883-1887.

线粒体DNA D-环区单核苷酸多态性与胃癌发病年龄的关系研究

1. 050011　河北省石家庄市，河北医科大学第四医院消化内科
2. 050011　河北省石家庄市，河北医科大学第四医院教务处
3. 050011　河北省石家庄市，河北医科大学第四医院风湿免疫科

通讯作者: 张瑞星
作者简介:
王英南，吴忱思，赵乐，等.线粒体DNA D-Loop区单核苷酸多态性与胃癌发病年龄的关系研究[J].中国全科医学，2022，25（15）：1883-1887. [www.chinagp.net] 作者贡献：王英南提出研究选题方向，进行文章的构思与设计，负责数据收集、采集、清洗和统计学分析；王英南、吴忱思、赵乐、张风宾负责撰写论文初稿；吴忱思、赵乐、张风宾、张韶辰负责数据收集、采集、清洗和统计学分析、绘制图表，论文修订；郭占军、张瑞星进行数据复核，负责文章的质量控制及审校，对文章整体负责；所有作者确认了论文的最终稿。
基金资助:
河北省卫生和计划生育委员会科研基金项目(20210508); 河北省医学科学研究重点课题计划(21377759D)

Abstract

Abstract:

Background

It is very difficult to contain persistently high incidence and mortality rates of gastric cancer. Moreover, the age at onset of gastric cancer is becoming earlier. However, there are few studies on the prediction of the age at gastric cancer onset.

Objective

To investigate the relationship between single nucleotide polymorphisms (SNPs) in mitochondrial displacement loop (D-Loop) and age at gastric cancer onset.

Methods

A total of 150 patients with confirmed gastric cancer by pathologically examination of biopsy samples taken during gastroscopy were recruited from Department of Gastroenterology, the Fourth Hospital of Hebei Medical University between July 2007 and December 2008. PCR was used to amplify the targeted fragment of peripheral blood mitochondrial DNA, and the mitochondrial D-Loop region was sequenced. Survival curves for patients with different ages of onset of gastric cancer were plotted by the Kaplan-Meier method, and compared by the Log-rank test. Cox regression analysis was used to identify factors associated with age of onset of gastric cancer.

Results

The age of onset of patients with different degrees of education was statistically significant (P<0.05) . Log-rank test revealed that patients with 153G genotype had earlier age of onset of gastric cancer than did those with 153A genotype〔 (48.0±5.3) vs (60.1±0.8) 〕 (χ²=7.757, P=0.005) . Multivariate Cox regression analysis indicated that the 153A/G polymorphism in mitochondrial D-Loop was a predictor of age at onset of gastric cancer〔HR=0.323, 95%CI (0.140, 0.745) , P=0.008〕.

Conclusion

The 153A/G polymorphism in mitochondrial D-Loop may be used as a novel predictor of the age at onset of gastric cancer. Analyzing the SNPs in mitochondrial D-Loop may contribute to early identification of gastric cancer.

Key words: Stomach neoplasms, DNA, mitochondrial, D-Loop, Polymorphism, single nucleotide, Age of onset, Sex factors

摘要：

背景

胃癌发病率和死亡率长期居高不下，防控形势严峻，且发病年龄逐渐年轻化，但目前关于胃癌发病年龄预测因素的研究报道较少。

目的

探讨线粒体DNA D-环区（D-Loop区）单核苷酸多态性（SNPs）与胃癌患者发病年龄之间的关系。

方法

选取2007年7月至2008年12月在河北医科大学第四医院消化内科经胃镜病理证实的胃癌患者150例为研究对象，提取胃癌患者外周血中的线粒体DNA，采用聚合酶链反应（PCR）扩增目的片段，并进行线粒体DNA D-Loop区测序。采用Kaplan-Meier法绘制胃癌患者发病年龄的生存曲线，比较采用Log-rank检验；采用多因素Cox比例风险回归分析探究胃癌患者发病年龄的影响因素。

结果

不同分化程度患者发病年龄比较，差异有统计学意义（P<0.05）。Log-rank检验结果显示，153G基因型患者的发病年龄〔（48.0±5.3）岁〕早于153A基因型患者〔（60.1±0.8）岁〕（χ²=7.757，P=0.005）。多因素Cox比例风险回归分析结果显示，线粒体DNA D-Loop区SNPs位点153A/G是预测胃癌患者发病年龄的影响因素〔HR=0.323，95%CI（0.140，0.745），P=0.008〕。

结论

线粒体DNA D-Loop区SNPs位点153A/G或许可以作为胃癌发病年龄的新型预测指标，通过分析线粒体DNA D-Loop区的多态性，可以帮助识别早发的胃癌患者。

关键词: 胃肿瘤, DNA，线粒体, D-Loop, 多态性，单核苷酸, 发病年龄, 性别因素

Yingnan WANG,Chensi WU,Yue ZHAO, et al. Single Nucleotide Polymorphisms in the Mitochondrial Displacement Loop and Age at Onset of Gastric Cancer[J]. Chinese General Practice, 2022, 25(15): 1883-1887. DOI: 10.12114/j.issn.1007-9572.2022.02.003.
王英南,吴忱思,赵乐等. 线粒体DNA D-环区单核苷酸多态性与胃癌发病年龄的关系研究[J]. 中国全科医学, 2022, 25(15): 1883-1887. DOI: 10.12114/j.issn.1007-9572.2022.02.003.

Figures/Tables 3

References 27

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特征		例数	<40岁	40~50岁	51~60岁	>60岁	χ²值	P值
性别							2.726	0.436
	男	107	5（4.7）	15（14.0）	31（29.0）	56（52.3）
	女	43	1（2.3）	4（9.3）	18（41.9）	20（46.5）
肿瘤大小（cm）							5.616	0.132
	≤4	37	1（2.7）	3（8.1）	8（21.6）	25（67.6）
	>4	113	5（4.4）	16（14.2）	41（36.3）	51（45.1）
肿瘤部位
	贲门	27	0	0	8（29.6）	19（70.4）	9.423	0.151
	胃体	74	3（4.1）	12（16.2）	27（36.5）	32（43.2）
	胃窦	49	3（6.1）	7（14.3）	14（28.6）	25（51.0）
分化程度							8.153	0.043
	低分化	76	6（7.9）	12（15.8）	24（31.6）	34（44.7）
	中~高分化	74	0（0.0）	7（9.5）	25（33.8）	42（56.8）
TNM分期（期）							3.405	0.333
	Ⅰ+Ⅱ	51	2（3.9）	3（5.9）	17（33.3）	29（56.9）
	Ⅲ+Ⅳ	99	4（4.0）	16（16.2）	32（32.3）	47（47.5）

特征		例数	<40岁	40~50岁	51~60岁	>60岁	χ²值	P值
性别							2.726	0.436
	男	107	5（4.7）	15（14.0）	31（29.0）	56（52.3）
	女	43	1（2.3）	4（9.3）	18（41.9）	20（46.5）
肿瘤大小（cm）							5.616	0.132
	≤4	37	1（2.7）	3（8.1）	8（21.6）	25（67.6）
	>4	113	5（4.4）	16（14.2）	41（36.3）	51（45.1）
肿瘤部位
	贲门	27	0	0	8（29.6）	19（70.4）	9.423	0.151
	胃体	74	3（4.1）	12（16.2）	27（36.5）	32（43.2）
	胃窦	49	3（6.1）	7（14.3）	14（28.6）	25（51.0）
分化程度							8.153	0.043
	低分化	76	6（7.9）	12（15.8）	24（31.6）	34（44.7）
	中~高分化	74	0（0.0）	7（9.5）	25（33.8）	42（56.8）
TNM分期（期）							3.405	0.333
	Ⅰ+Ⅱ	51	2（3.9）	3（5.9）	17（33.3）	29（56.9）
	Ⅲ+Ⅳ	99	4（4.0）	16（16.2）	32（32.3）	47（47.5）

变量	B	SE	Wald χ²值	P值	HR	95%CI
性别	0.121	0.185	0.427	0.513	1.129	（0.785，1.622）
mtDNA D-Loop区SNPs位点153	-1.130	0.426	7.027	0.008	0.323	（0.140，0.745）

变量	B	SE	Wald χ²值	P值	HR	95%CI
性别	0.121	0.185	0.427	0.513	1.129	（0.785，1.622）
mtDNA D-Loop区SNPs位点153	-1.130	0.426	7.027	0.008	0.323	（0.140，0.745）

Single Nucleotide Polymorphisms in the Mitochondrial Displacement Loop and Age at Onset of Gastric Cancer

线粒体DNA D-环区单核苷酸多态性与胃癌发病年龄的关系研究

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