Abstract: Objective　To investigate the stability and reliability of serum thyroid hormone levels measured by the Roche automatic electrochemiluminescence immunoassay system and to compare the differences in these levels in different strains of rats and mice.Methods　From November 2016 to March 2017，we selected 60 Kunming mice and 30 SD rats aged 7–8 weeks；30 Kunming mice aged 7–8 weeks （randomly assigned to the experimental thyroid hormone elevation group，experimental thyroid hormone reduction group and normal thyroid hormone group，10 in each group） and 30 SD rats aged 7–8 weeks （randomly assigned to the experimental thyroid hormone elevation group，experimental thyroid hormone reduction group and normal thyroid hormone group，10 in each group）；outbred strains of SD rats，inbred BN rats，outbred Kunming mice，inbred C57BL/B6N mice age 10 weeks，20 in each strain.The automatic electrochemiluminescence immunoassay system was used to detect TT3，TT4，FT3 and FT4.Results　The within-assay coefficients of variation （CVs） of serum thyroid function indicators in Kunming mice and SD rats were 0.697%–1.853% and 0.946%–1.253%，respectively，while the inter-assay CVs were 1.389%–2.670% and 1.150%–2.834%，respectively.The levels of TT3，TT4，FT3，and FT4 in the experimental thyroid hormone elevation group of Kunming mice and SD rats were higher than those in the normal thyroid hormone group，while those in the experimental thyroid hormone reduction group were lower than those in the normal thyroid hormone group （P<0.05）．The CVs of serum thyroid function indicators were > 5.000% in all strains of rats and mice，while there was a tendency for inbred strains' serum thyroid function indicators to outperform those of the outbred strains.Conclusion　The Roche automatic electrochemiluminescence immunoassay system shows stability in the detection of thyroid hormone levels in rats and mice.It distinguished the differences in thyroid functions in the experimental thyroid hormone elevation group，experimental thyroid hormone reduction group and normal thyroid hormone group.

Key words: Thyroid hormones, Roche automatic electrochemiluminescence immunoassay system, Rat serum

摘要： 目的　探讨罗氏全自动电化学发光免疫分析系统检测大、小鼠血清甲状腺激素水平的稳定性、可靠性，以及比较不同品系大、小鼠血清甲状腺激素水平的差异。方法　2016年11月—2017年3月，选取7~8周龄昆明小鼠60只和SD大鼠30只；7~8周龄昆明小鼠30只（随机分为实验性甲状腺激素增多组、实验性甲状腺激素减少组和甲状腺功能正常组，各10只），7~8周龄SD大鼠30只（随机分为实验性甲状腺激素增多组、实验性甲状腺激素减少组和甲状腺功能正常组，各10只）；10周龄远交系SD大鼠、近交系BN大鼠、远交系昆明小鼠、近交系C57BL/B6N小鼠各20只。采用全自动电化学发光免疫分析系统检测总三碘甲状腺原氨酸（TT3）、总甲状腺素（TT4）、游离三碘甲状腺原氨酸（FT3）、游离甲状腺素（FT4）。结果　昆明小鼠、SD大鼠血清甲状腺功能指标批内变异系数分别为0.697%~1.853%、0.946%~1.253%，昆明小鼠、SD大鼠血清甲状腺功能指标批间变异系数分别为1.389%~2.670%、1.150%~2.834%。昆明小鼠、SD大鼠实验性甲状腺激素增多组TT3、TT4、FT3、FT4水平高于甲状腺功能正常组，实验性甲状腺激素减少组TT3、TT4、FT3、FT4水平低于甲状腺功能正常组（P<0.05）。各品系实验动物血清甲状腺功能指标变异系数均>5.000%，近交系实验动物血清甲状腺功能指标集中趋势优于远交系。结论　罗氏全自动电化学发光免疫分析系统检测大、小鼠甲状腺激素水平的结果稳定性较好，能够较好地区分实验性甲状腺激素增多组、实验性甲状腺激素减少组及甲状腺功能正常组甲状腺功能的差异。

关键词: 甲状腺激素类, 罗氏全自动电化学发光免疫分析系统, 鼠血清