Abstract: Objective　To explore the expression of calcium activated chloride channel （CaCCs） TMEM16A in rat atrial and ventricular tissues,so as to provide a new therapeutic target for clinical treatment of myocardial ischemia,arrhythmia and heart failure.Methods　From October 2014 to September 2015，18 clean grade Sprague-Dawley rats were given 1 week of adaptive feeding,then,the heart was taken out after intraperitoneal injection of 10% chloral hydrate,and the atrial and ventricular tissues were isolated.The expression of TMEM16A and its mRNA in atrial and ventricular tissues were detected by Western blotting methods and real-time fluorescence quantitative reverse transcription PCR （RT-qPCR）,respectively.Results　The expression levels of TMEM16A in the rat atrial tissues were higher than those in the ventricular tissues〔（0.46±0.02） vs. （0.33±0.03）,P<0.01〕.The expression levels of TMEM16A mRNA in the rat atrial tissues were higher than those in the ventricular tissues〔（3.55±0.17） vs. （1.00±0.03）,P<0.01〕.Conclusion　TMEM16A CaCCs was expressed in both atrial and ventricular tissues of rats,and the expression level in the atrial tissue was higher than that in the ventricular tissue.

Key words: TMEM16A, Calcium-activated chloride channels, Heart atrial, Heart ventricles

摘要： 目的　探究钙激活氯通道（CaCCs）TMEM16A在大鼠心房和心室组织中的表达情况，为临床治疗心肌缺血、心律失常以及心力衰竭等疾病提供新的治疗靶点。方法　2014年10月—2015年9月，将18只清洁级Sprague-Dawley大鼠适应性饲养1周，采用10%水合氯醛溶液腹腔注射麻醉后取心脏，分离心房和心室组织进行实验。采用Western blotting法和实时荧光定量反转录PCR（RT-qPCR）法分别检测大鼠心房和心室组织中TMEM16A及其mRNA水平。结果　大鼠心房和心室组织TMEM16A水平分别为（0.46±0.02）和（0.33±0.03），心房和心室组织TMEM16A mRNA水平分别为（3.55±0.17）和（1.00±0.03），大鼠心房组织中TMEM16A及其mRNA水平高于心室组织（P<0.01）。结论　CaCCs TMEM16A在大鼠心房和心室组织中均有表达，其在心房组织中的水平高于心室组织。

关键词: TMEM16A, 钙激活氯通道, 心房, 心室