Abstract: Objective　To detect serum anti-keratin antibody （AKA）,anti-cyclic citrullinated peptide （Anti-CCP） antibody and rheumatoid factor （RF） in rats type Ⅱ collagen-induced arthritis （CIA）,and to analyze whether they are similar with serum immunology of human rheumatoid arthritis （RA）.Methods　After one-week adaptive feed,among 15 healthy female Wistar rats （SPF level） aged 7 to 8 weeks,5 were selected as blank control group by random number table method,and 10 for building type Ⅱ CIA model from November 2014 to March 2015,and finally 8 rats’ modeling was successfully built（CIA model group）.After successful modeling,femoral artery blood of rats in CIA model group and blank control group was collected,and serum was routinely separated,serum AKA of rats in two groups and Anti-CCP antibody and RF-IgM antibody were detected by indirect immunofluorescence （IIF） method and enzyme-linked immunosorbent assay （ELISA） method respectively;40 g/L paraformaldehyde fixation was used as pathological specimens to isolate synovium of knee joint.The pathological specimens were conducted with HE staining.Results　Serum AKA of 5 cases （5/8） were positive in CIA model group,all were negative in blank control group.There was no significant difference in levels of serum Anti-CCP antibody and RF-IgM antibody between rats in blank control group and CIA model group （P>0.05）.Pathological HE staining observation of joint synovium of rats in blank control group presented clear arrangement of synovial cells,normal histology structure,no hyperplasia,no significant accumulation and infiltration of inflammatory cells;pathological HE staining observation of joint synovium of rats in CIA model group showed disordered arrangement of synovial cells,capillary proliferation,significant synovial hyperplasia,synovial infiltration of large amount of inflammatory cells.Conclusion　Serum of CIA rats group in shows positive AKA,which is similar with serum immunology of human RA,and suits for basic research of RA.

Key words: Arthritis,rheumatoid, Rats,Wistar, Rheumatoid factor, Anti-keratin antibody, Anti-cyclic citrullinated peptide antibody

摘要： 目的　对Ⅱ型胶原诱导性关节炎（CIA）大鼠血清抗角蛋白抗体（AKA）、抗环瓜氨酸肽（Anti-CCP）抗体及类风湿因子（RF）进行检测，并分析其与人类类风湿关节炎（RA）的血清免疫学是否相似。方法　2014年11月—2015年3月，15只SPF级7~8周健康雌性Wistar大鼠适应性喂养1周后，采用随机数字表法选取5只作为空白对照组，10只用于建立CIA模型，最终8只造模成功（CIA模型组）。造模成功后，采集CIA模型组和空白对照组大鼠股动脉血，常规分离血清，分别采用间接免疫荧光（IIF）法检测两组大鼠血清AKA，酶联免疫吸附实验（ELISA）法检测Anti-CCP抗体和RF-IgM抗体；分离膝关节滑膜，用40 g/L多聚甲醛固定作为病理标本，进行HE染色。结果　CIA模型组大鼠血清AKA阳性5例，空白对照组均阴性。空白对照组和CIA模型组大鼠血清Anti-CCP抗体及RF-IgM抗体水平比较，差异均无统计学意义（P>0.05）。空白对照组大鼠关节滑膜病理HE染色可见滑膜细胞排列清晰，组织学结构正常，未见增生，无明显炎性细胞聚集和浸润；CIA模型组大鼠关节滑膜病理HE染色可见滑膜细胞排列紊乱，毛细血管增生，滑膜增生明显，滑膜下大量炎性细胞浸润。结论　CIA大鼠血清中出现AKA阳性，与人类RA的血清免疫学类似，适用于RA的基础研究。

关键词: 关节炎,类风湿, 大鼠,Wistar, 类风湿因子, 抗角蛋白抗体, 抗环瓜氨酸肽抗体